Naturally occurring peptides are made up of 2 to 50 amino acids and function in almost all biological processes. From bioactive peptides targeting protein-coupled receptors, to hormones, neurotransmitters and antimicrobial peptides, it is evident that these ‘strings’ of amino acids have been carefully moulded by nature for a variety of specific purposes.
Through scientific innovation, peptides are now seen as influential tools in medicine, cosmetics and food industries. Therefore, the need to rapidly select for peptides with desirable properties has never been greater.
Peptide libraries are highly advantageous in the identification of target peptides and accommodate a whole spectrum of applications. Such examples are their use in the discovery of pharmaceutical peptides; investigations into signalling pathways, protein-protein interactions, protein characterization and mapping domains; and to study consensus sequences and critical residues in enzymes. Peptide libraries can even lead to the discovery of potential biomarkers in diseases states within clinical trials.
Biosynth can be relied upon to create high quality peptide libraries to support these critical scientific applications, whether it be in investigations into T-cell activating peptides, vaccine development or the study of antibody response and binding in specific infections.
Peptides make excellent drug candidates due to their ability to bind to their target molecules with high specificity and affinity. This and the fact that they can be degraded naturally in the human body into amino acids, means that the risk of toxicity and severe side effects is greatly reduced. The stability of a peptide is another pharmaceutical element that must be considered, as well as how easily they can permeate tissues and cell membranes.
In the drug discovery world, it is imperative that the peptide with the most applicable characteristics is selected, and this can be achieved using peptide library screening. At Biosynth, our peptide libraries and high throughput screening services can support you every step of the way. We can also further enhance the qualities of your selected peptides through our expansive range of peptide modifications: Biotin, Fluorescein, highly constrained CLIPS, C-terminal and many more.
Peptide Libraries can also contribute to epitope mapping, helping scientists gain insight into how specific residues of an antigen (an epitope), bind and interact with their complementary antibody. This is crucial in a clinical context as it can help in the development of antibody-based therapeutics against infectious and autoimmune diseases.
Biosynth’s Epitope Mapping Work Flow
We at Biosynth can provide you with a reliable epitope mapping service. Visit our Epitope Mapping page on our website for more information.
T-cells are fundamental to the adaptive immune response. In response to peptide antigens presented by major histocompatibility complex (MHC) molecules, mature T-cells express unique αβ T cell receptors and are activated. Excessive activation of T-cells is prevalent in autoimmune diseases such as asthma, immune bowel diseases and psoriasis. Consequently, it is of interest to investigate and identify antigenic peptides which may be involved in T-cell activation. Again, peptide libraries and high throughput screening of these libraries can be of benefit to this area of research.
Biosynth is proud to offer to our customers novel peptide library high throughput screening solutions through our custom peptide libraries. As global leaders in this peptide library technology our team of peptide experts, can synthesize 96 different peptides in a 96 well plate format, featuring a range of yields, purities and peptide lengths.
Synthesizing peptide libraries rapidly, cost-effectively and to a high standard is our specialism and we test each individual plate for accuracy and can support a variety of scientific application such as their use in peptide libraries, protein characterization, epitope mapping and additional high throughput screening processes. We can also offer linear or peptides with post-translation modifications at your request, for example Fluorescein, Biotin and highly constrained CLIPSTM.
Biosynth can further provide our customers with crude libraries with heavy/light tryptic peptide fragments for use in MRM and Quantitative Proteomics.
Our Custom Peptide Libraries can offer our customers:
Two tiers of analysis are available for our custom peptide libraries (see below) however only tier two is available for our crude libraries containing heavy/light tryptic peptide fragments.
It is important to note that peptide libraries are intended for High Throughput Screening and the purities of the peptides may vary between 25-95%. Although customers can request a range of peptide length our service is more suitable for peptide of 10-25 amino acids in length. Furthermore, the failure rate per plate is roughly <5%, however this may increase with modified sequences, longer peptides and hydrophobic sequences.
Please visit this web page for more information or contact us for more information on how our custom peptide services can support you and your project.
Keyword: Peptide APIs